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Untargeted metabolomics analysis Fecal specimens (50 mg) were homogenized with a methanol-acetonitrile-water (2:2:1, v/v/v) extraction solvent using an ultrasonic-assisted extraction method, followed by protein precipitation and centrifugal separation

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Biochemistry 56:32993306 Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K (2000) Cohesins binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins