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Statistics All results were represented as means standard error of the mean (SEM)

The largest increase in binding was observed upon substitution of the aliphatic ether by a benzyl ether group ( 32 ), resulting in a five-fold increase in affinity as measured by the R2KD assay

Comparison of impact of ACore on presynaptic terminal volume and number To investigate the impact of the ACore on the corresponding presynaptic side of the synapse, the volume, surface area, and count per region of interest (ROI) of spine-innervating nerve terminals were assessed in primary hippocampal neuronal cultures using the synaptic vesicle marker, synaptophysin, apposed to PSDs in identified spines (Fig