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Disclosures: Tony (Chun-Cheng) Chiang: Nothing to Disclose, Donghun Shin: Nothing to Disclose, Shuchang Liu: Nothing to Disclose, Satdarshan (Paul) Monga: Alnylam: Grant/Research Support, Alnylam: Consultant, Surrozen: Independent Contractor, Fog Pharmaceuticals: Grant/Research Support, Mermaid Bio: Advisor, Vicero: Advisor, AntlerA: Advisor, Genentech: Speaking and Teaching, Surrozen: Consultant, Kari Nejak-Bowen: Surrozen, Inc.: Consultant 2075 3D RECONSTRUCTION OF BILIARY SYSTEM IN THE BIOENGINEERED LIVERS USING DECELLULARIZED SCAFFFOLDS Hiroshi Horie 1 Ken Fukumitsu 1 Kentaro Iwaki 1 Fumiaki Munekage 1 Kenta Makino 1 Katsuhiro Tomofuji 1 Hidenobu Kojima 2 Elena Uebayashi 2 Satoshi Ogiso 2 Takamichi Ishii 2 Etsuro Hatano 2 , 1 Department of Surgery, Graduate School of Medicine, Kyoto University, 2 Deparment of Surgery, Graduate School of Medicine, Kyoto University Background: The function of bioengineered livers generated by recellularization of cells that construct the liver such as hepatocytes, cholangiocytes and vascular endothelial cells has been investigated, but the reconstruction of functional biliary system in liver scaffolds has not been achieved

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