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Nociceptin and its receptor in rat dorsal root ganglion neurons in neuropathic and inflammatory pain models: Implications on pain processing

By detecting the appearance of the colored quinones on a TECAN infinite M200 absorption curves were recorded at different substrate molarities in a total volume of 200 l containing 50 mM sodium phosphate buffer at pH 6.0, 2 mM SDS and variable amounts of enzyme (Table S7) at 25 C