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Many studies have been done to prove that hydroxocobalamin is a better form of vitamin B12, however, due to increased cost it is not used as often

Ascorbic Acid: Biochemistry and Biomedical Cell Biology

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For purification, harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice