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The effect of tau K677 lactylation on ferritinophagy and ferroptosis in Alzheimers disease

Co-cultured cells were collected in one 15 ml falcon tube and lysed by RIPA buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) containing protease inhibitor cocktail (Sigma-Aldrich) and PMSF (Sigma-Aldrich) with 1 M Sodium-Orthovanadate pH 10(Sigma-Aldrich)