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Materials and methods Materials Nunc EasYFlask 225 cm 2 (T225) cell culture flasks, Corning Costar six-well clear tissue culture-treated flat bottom plates, Falcon 50 mL conical centrifuge tubes, Basix microcentrifuge tubes, Invitrogen Countess cell counting chamber slides, Nunc 25 mL serological pipettes, Greiner Bio-One CELLSTAR 24-well cell culture plates, Corning 96-well solid white non-sterile microplates, 9 and 5 glass Pasteur pipets, Gibco trypsinEDTA (0.25%) phenol red, Gibco DMEM/F-12 with l-glutamine and phenol red, Corning penicillinstreptomycin (pen-strep) solution (10,000 IU penicillin, 10,000 g/mL streptomycin), and Corning regular fetal bovine serum (FBS) were purchased from Fisher Scientific (Pittsburgh, PA, USA)

Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation

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The osmolarity is 0.This preparation is designed solely for parenteral use only after addition to drugs that require dilution or must be dissolved in an aqueous vehicle prior to injection.Sterile Water for Injection contains no bacteriostatic, antimicrobial agent or added buffer and is supplied only in single dose containers to dilute or dissolve drugs for injection.For IV injection, add sufficient amount to a solute to make an approximately isotonic solution

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