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A DNA fragment encoding the flipped AAGAB variant (amino acids 258 to 315, 188 to 247, and 1 to 187) was synthesized by Integrated DNA Technologies (IDT) and subcloned into the Hind III and Xba I sites of the p3xFLAG-7.1 vector (Sigma-Aldrich, catalog no

Recognition of tRNAs by aminoacyl-tRNA synthetases

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