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104 DFO is derived from Streptomyces pilus and is approved for managing iron overloadrelated diseases such as hemochromatosis, thalassemia, and sickle cell anemia, as well as other chronic iron overloadinduced conditions such as blood transfusion

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Importantly, iodoacetamide (IAA, a known thiol-reactive compound) was not able to oxidize roGFP when cells were exposed to non-lethal or subtoxic concentrations of this compound, indicating that an electrophilic attack on roGFP is not sufficient to cause a spectral change consistent with its oxidation (Supplementary Fig

Secondary bile acids Secondary bile acids are generated by the gut microbiota through the conversion of primary bile acids, including lithocholic acid (LCA), deoxycholic acid (DCA), and its derivative 3-oxoLCA