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doi: 10.3389/FIMMU.2013.00105/XML/NLM 129 Ruiz-PablosMPaivaBZabaletaA

FAD absorption spectroscopy To assess the presence of protein-bound FAD, 100 g of recombinant WT or D285N mutant FSP1 protein or 10 g of free FAD control was resuspended in a total volume of 200 l in 50 mM sodium phosphate pH 7.4