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Redox activity of cells in response to their environment has important implications on periodontal disease

Western blotting After treatment with bortezomib (4 nM), UroA (15 M), their combination, or vehicle (as a control), cells were lysed and cleared by centrifugation (14,000 g , 4 C, 5 min), and the protein concentration was determined with the Quick Start Bradford 1 Dye Reagent (Bio-Rad Laboratories, Hercules, CA, USA) on the Epoch microplate reader

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