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In both dogs and cats, clinical signs of water deficiency include the loss of elasticity of the skin, reduced saliva secretion, reduced appetite, reduced blood volume, impaired blood circulation, the formation of bladder and kidney stones, and the dysfunction of tissues

For the BCA assay, 50 L of the supernatant were mixed with 950 L of the BCA solution, incubated at 37 C for 15 min, and analyzed using a spectrophotometer (562 nm)

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These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione

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