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Stock solutions were prepared by dissolving luminol (2.5 mM) in 50 mM borate buffer (H 3 BO 3 , pH 9) and the peroxidase substrate HPI (2.5 mM) in PBS with 30% DMSO

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doi: 10.3389/fendo.2020.00575 238 ChangZPDengGFShaoYYXuDZhaoYNSunYFet al

Using this approach, it was observed that erastin-induced death was not consistently modulated by inhibitors of caspase, cathepsin or calpain proteases (z-VAD-fmk, E64d or ALLN), RIPK1 (necrostatin-1), cyclophilin D (cyclosporin A) or lysosomal function/autophagy (bafilomycin A1, 3-methyladenine, chloroquine), compounds known to inhibit various forms of apoptosis, necrosis and autophagic cell death ( DFO, the anti-oxidant trolox, the MEK inhibitor U0126 and, to a weaker extent, the protein synthesis inhibitor cycloheximide (CHX), all rescued from erastin-induced death in HT-1080, BJeLR and Calu-1 cells (FIG

When comparing more than two groups, data were statistically analyzed by one-way ANOVA followed by a Dunnett multiple comparisons test