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T2-weighted images were acquired with Refocused Echoes (RARE) sequence (repetition time (TR) = 3,500 ms, effective echo time (TE) = 45 ms, rapid acquisition and relaxation enhancement (RARE) factor = 8, voxel size = 4507676 m)

Typical use cases include: Routine subculture and passaging of adherent mammalian cells Gentle single-cell dissociation of hESCs, iPSCs and other sensitive lines Sample preparation for flow cytometry and FACS analysis Analysis of cell-surface markers where epitope integrity matters Cell migration, proliferation and apoptosis assays Quiescence assays by serum starvation and oncogene transfection studies Tumor cell and neural crest cell migration assays Production scale-up in bioreactor workflows For routine work, apply approximately 10 ml of Accutase per 75 cm2 of culture surface and incubate for 510 minutes at room temperature

Dong Y, et al

Chen H-Y, Xiao Z-Z, Ling X, Xu R-N, Zhu P, Zheng S-Y