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Effect of Bacillus subtilis C-3102 on bone mineral density in healthy postmenopausal Japanese women: a randomized, placebo-controlled, double-blind clinical trial

Quantitative real-time PCR Total RNA from INS-1E cells and the INS-1E-derived 2A -adrenergic receptor knockout cell line were isolated using the RNeasy Plus micro Kit (Qiagen, Valencia, CA)