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No muscle fiber atrophy was noted

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After TCA uptake for 2 min, cells were washed in deoxycholic-acid-free and Na + -free (to avoid TCA reuptake by ASBT) buffer for 5 min or 10 min, and TCA in the cell lysate and the wash buffer were measured

Additional laboratory investigations examine cellular methylation-related pathways and NAD-associated signaling systems , which play a role in cellular energy dynamics and metabolic pathway coordination in controlled experimental settings

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