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For immunofluorescence, section were incubated with antibody against TSC2 (D93F12) (cell signaling, 1100) and with Alexa Fluor 488-labeled goat anti-rabbit IgG (H+L) secondary antibody (Molecular Probes

B12 shots take minutes and cost less per session, while IV infusions require 30 to 60 minutes and carry higher price points that reflect their expanded nutrient delivery and medical oversight