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The UPLC system was operated with a gradient elution program consisting of water with 0.1% formic acid (mobile phase A) and methanol (mobile phase B)

As a result, different organisms in the same polluted environment may exhibit vastly different levels of metal accumulation, underscoring the importance of selecting appropriate sentinel species for assessing heavy metal burdens in aquatic ecosystems

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Also although this overview focuses on E.coli , protein expression and purification from the other commonly used expression systems are mentioned and apart from cell breakage methods, the protein purification methods and strategies are essentially the same