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Acute toxicity studies in animals, if extrapolated to humans, implicate a single dose of 10-15 grams necessary to produce significant liver toxicity, although several human case reports have shown toxicity at doses less than 4 grams per day (likely due to having one or more risk factors for sensitivity to toxicity) (Amar 2007)

Chen Y-H, Yang S-F, Yang C-K, Tsai H-D, Chen T-H, Chou M, Hsiao Y-H (2021) Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells

A mild deficiency may not cause any symptoms

& Camarero, S

Successful production of intrathyroidal human T cell hybridomas: evidence for intact helper T cell function in Graves disease