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doi: 10.1007/s007020070050

doi: 10.3390/nu11102389 114 DAngeloS

delivered via intravenous therapy

In addition, following AS-IV administration, the activity of the mediators involved in G2/M cell cycle arrest, including p21, p53, p-p53 and p-histone H3, markedly decreased, indicating that AS-IV can target CKD-related G2/M cell cycle change

After washing with PBS, cells were fixed in 4% paraformaldehyde for 30 min at room temperature, washed twice with PBS, and blocked with 1% BSA/PBS for 30 min