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Quantification was done in at least 10 fields for each condition and the proportion of dead cells was calculated as a percentage of total cells expressing the WT or WD-associated variants

CCK-8 results were similar to those observed with RAW264.7 macrophages, with cell death observed in all five cell types (BMDM, THP-1, U937, A549, and BEAS-2B), with a much higher death rate of epithelial cells (A549 and BEAS-2B) (Fig