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Collectively, these multifaceted interactions emphasize the critical roles played by gut microbiota in the etiology and progression of hypothyroidism, underscoring the therapeutic potential of targeted microbiota interventions, including probiotics, prebiotics, dietary adjustments, and lifestyle modifications, to restore microbiome equilibrium and mitigate hypothyroid pathology

The serotonin level was estimated using the method described by (44)

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Robinson MK, Rodrick ML, Jacobs DO, Rounds JD, Collins KH, Saporoschetz IB, Mannick JA, Wilmore DW