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Highly multiplexed CosMx spatial transcriptomics tissue processing, staining, imaging, and analysis FFPE TMA processing, staining, imaging, and cell segmentation were performed as previously described by Nanostring and data were analyzed at UCSF 39

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Moreover, gastrointestinal symptoms often reported by individuals with ME/CFS underscore the potential gut-brain axis involvement in disease manifestation [8, 13]

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