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Isolation of mouse primary adipocytes and lipolysis assay Epididymal fat was excised, minced and digested with 0.2 % ( w/v ) collagenase type I (Life Technologies) for 30 min at 37 C under shaking, as previously described 29

Lastly, choline has also been shown to specifically aid with memory, and to support the maintenance of a healthy nervous system.1314 Food sources of choline include peanuts, soybeans, wheat, chicken, fish, beef, cauliflower, eggs, and lettuce