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This antitumor effect could be reversed by aspartate supplementation [26], reinforcing that the presence of other metabolites (in this case, another amino acid) in the TME can influence the effects of a single one in tumor biology

10.1083/jcb.201006121 117 LiH.KorennykhA

The mixture was incubated at 37 C for 530 min, and then at 30 C before substrate introduction

Trophic factor-induced intracellular calcium oscillations are required for the expression of postsynaptic acetylcholine receptors during synapse formation between Lymnaea neurons