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the Fmoc amino acid (0.5 mmol) and coupling agent (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), 0.45 mmol) were dissolved in DMF (1.5 mL) and base (4-methylmorpholine (NMM), 1 mmol) added

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By inhibiting the cystine transporter (xCT), erastin inhibits the uptake of cystine

Briefly, to separate epidermis from dermis, whole skin from mice at postnatal day (P)0-P3 were incubated in a solution of 5 mg/ml Dispase II (#D4693, Sigma-Aldrich, USA) diluted in RPMI medium (Gibco, USA) supplemented with 10% fetal calf serum (FCS, #S0615, Sigma-Aldrich, USA), penicillin (100 U/ml), streptomycin (100 g/ml), 100 nM sodium pyruvate, and 10 mM non-essential amino acids (all Gibco, USA) at 4 C overnight

Respect the pharmacokinetics