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For in vivo experiments, total RNA was extracted from the rat retina using the miRNeasy mini kit (QIAGEN, Hilden, Germany) and then reverse-transcribed into cDNA using the SuperScript III First-Strand Synthesis System (Thermo Fisher Scientific, Waltham, MA, USA)

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However, published phase 3 papers focused primarily on total weight and glycemic control without reporting detailed body composition data comparing lean mass preservation between treatment groups

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