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Assays to measure serum concentrations of insulin Mice were anesthetized using 2% isoflurane mixed with oxygen

During stressful circumstances, the distinctive oxidative burst due to catalytic activities of specified ROS-generating enzymes such as nicotinamide adenine dinucleotide phosphate (NADPH) oxidases or cell wall peroxidases (PODs) lead to vast ROS accumulation in the affected cells [91]

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Similarly, the involvement of oxidative stress in sepsis, which disrupts redox signaling, leading to molecular damage, has been highlighted (172)