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Model building and refinement The initial structure model of hTauT was computationally generated using AlphaFold3 (AlphaFold Protein Structure Database, #AF-P31641-F1) 58 and then fitted into the cryo-EM map using UCSF Chimera X 59 and rebuilt manually using Coot 60

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Browse all Methylation & MTHFR education Medical and Editorial Standards Medical review process: This article was reviewed for medical accuracy, scientific clarity, evidence alignment, and appropriate discussion of genetics, medications, supplements, biomarkers, and health-related claims