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The key principle is that any combination therapy should be initiated and monitored under close medical supervision, with dose adjustments made based on glucose monitoring results and individual patient response

For intracellular staining with a PE-CY7 anti-IL-17 mAb (506921, BioLegend, USA) and a PE anti-IFN- mAb (163503, BioLegend, USA), the cells were treated with phorbol 12-myristate 13-acetate/ionomycin for 4 h, including GolgiPlug (BD Biosciences)

A hyperpronated foot could also contribute to the injury mechanism

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N Engl J Med 316:775779