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Healthy participants were recruited ( n = 9), under the following criteria: (1) age 20 years old

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While B-12 supplements and foods fortified with the vitamin are readily available, vitamin B-12 shots are only available by prescription, so a clinical diagnosis is always necessary

Immunoblots show SUMM2 using -HA antibodies (top panel), and TPR14 TPR or TPR5G using -FLAG antibodies (middle panel) with CBB staining of RBC as the loading control (bottom panel)