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References: [9] [2008] The human tri-peptide GHK and tissue remodeling [11] [2015] GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration [12] [2014] GHK and DNA: Resetting the Human Genome to Health [13] [2018] Medical Professional Monograph GHK-Cu [14] [2018] A Molecular Depot: Human Serum Albumin Carries Essential Cu and Zn in Serum [16] [2020] The potential of GHK as an anti-aging peptide [17] [2017] Topical Peptide Treatments with Effective Anti-Aging Results [19] [2022] () Skin permeability, a dismissed necessity for anti-wrinkle peptide performance

Tissues for immunostaining underwent deparaffinization followed by antigen retrieval for 20 min at 100 C with Leica Biosystems ER2 solution (pH9, AR9640) and endogenous peroxidase activity blocking with H 2 O 2

doi: 10.1001/jamainternmed.2022.4969

In agreement with this hypothesis, ectopic expression of HpaA deleted in the C-terminal HpaB-binding site did not significantly interfere with pathogenicity of strain 8510 (Supplementary Figure S2)