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Concerning the application, TD-DCS is an emerging field, with, to date, just few papers published

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Research has demonstrated that GHK-Cu treatment increases production of collagen (particularly type I and type III collagen, the predominant structural collagens in skin and connective tissues), glycosaminoglycans (including hyaluronic acid, which provides hydration, volume, and biochemical signaling in skin), decorin (a small proteoglycan involved in collagen fibril organization and growth factor regulation), and other matrix molecules critical for tissue structure and function

Blood was collected via jugular vein catheters, centrifuged to obtain serum, and stored at -20C