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Behav Brain Res (2018) 336:329

After blocking, washing, incubation with primary antibody, and washing, the membranes were incubated with a secondary antibody

Bratbak, G., Egge, J

Based on available biochemical, clinical and epidemiological studies, the current RDA for ascorbic acid is suggested to be 100120 mg/day to achieve cellular saturation and optimum risk reduction of heart diseases, stroke and cancer in healthy individuals