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In order to get rid of contaminating metal ions in the buffer, the mobile phase was eluted through the Chelex100 Chelating Ion Exchange resin (Sigma, Merck KGaA, Darmstadt, Germany) prior to liquid chromatographic separation

The concentration of MDA was determined with a colorimetric assay, using thiobarbituric acid (TBA)

Bioengineered 12(2):1092410934 Ghiasian M, Khamisabadi F, Kheiripour N, Karami M, Haddadi R, Ghaleiha A, Taghvaei B, Oliaie SS, Salehi M, Samadi P, Ranjbar A (2019) Effects of crocin in reducing DNA damage, inflammation, and oxidative stress in multiple sclerosis patients: a double-blind, randomized, and placebocontrolled trial

Changes to the treatment plan are made based on these checks to help patients get the best results

Future Research Directions: While our study sheds light on the diverse components of breast milk, numerous questions remain unanswered