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Description

Methods Pathol

10.1038/s41556-019-0305-6 Nat

Cell 174 , 780784 (2018)

Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)