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The mRNA levels of CCL2 (C-C Motif Chemokine Ligand 2), CCL11 (C-C Motif Chemokine Ligand 11), CSF2 (Colony Stimulating Factor 2), CXCL10 (C-X-C Motif Chemokine Ligand 10), HGF (Hepatocyte Growth Factor), IL1RA (Interleukin 1 Receptor Antagonist), IL6 (Interleukin 6), IL10 (Interleukin 10), IL31 (Interleukin 31), LIF (LIF Interleukin 6 Family Cytokine), and NGF (Nerve Growth Factor) were quantified using specific primers in SYBR Green (Thermo Fisher) or TaqMan (Thermo Fisher) platforms following the manufacturers instructions

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Common anemia categories include: Iron deficiency anemia Thalassemia (D56 codes) Sickle cell disorders (D57 codes) Anemia of chronic disease (often linked to CKD, cancer, or autoimmune conditions) Hemolytic anemia (where red blood cells are destroyed faster than theyre produced) Aplastic anemia (bone marrow failure) Vitamin B12 deficiency anemia Folate deficiency anemia Blood loss anemia Different sources group anemia types differently, so the 7 types of anemia phrasing seen in search results is an oversimplification

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