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Studies where GHK was used for the healing of bone fractures in rats used a mixture of small molecules (Gly-His-Lys (0.5 g/kg), dalargin (1.2 g/kg) (an opioid-like synthetic drug), and the biological peptide thymogen (0.5 g/kg) (L-glutamyl-L-tryptophan)) to heal bones

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Following, trans-enoyl-CoA is hydrated across the double bond to beta-hydroxyacyl-CoA, just like fumarate is hydrated to malate

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