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The mean fluorescence intensity for ROS-responsive hydrogel with monomer copper peptides M-CuP (G/M-CuP) and G/D-CuP groups decreased by 71% and 63%, respectively, in comparison to the NC group (Supplementary Fig

Proteolytic stability assay To evaluate the proteolytic stability of peptides against chymotrypsin, the peptides were dissolved to 1 mg/mL and incubated with chymotrypsin (1 mg/mL) at 37C for different times (0, 1, 2, 4, 8, and 16 h)

Research Use Limitation Used solely for laboratory and in vitro research Not approved for human consumption Not approved for veterinary use Not intended for diagnostic purposes Not intended for therapeutic applications Not permitted for clinical administration For research use only Independently analysed by Liquilabs s.r.o

In biological systems or culture media containing trace amounts of copper, GHK will spontaneously chelate the ion to form the GHK-Cu complex

10.1038/nn.2358 8 AsliA.Higazy-MreihS.Avital-ShachamM.KosloffM