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In addition, it was also confirmed that lactate can induce the formation of MUFAs through the HCAR1/MCT1-SREBP1-SCD1 pathway, which contributes to the resistance of liver cancer cells to ferroptosis induced by oxidative stress (Zhao et al., 2020) (Figure 3)

Recent regulatory guidance documents, such as the 2025 Australian Therapeutic Goods Administration (TGA) guidance on the quality of listed probiotic medicines and broader discussions on LBPs from 2020, underscore the necessity for strain-level identification, validated enumeration techniques, and explicit labeling of potency and storage conditions (570, 571)

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For quantifying lipid peroxidation, 95,000 cells were seeded into 12-well plates and treated as indicated