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Description

Allergy 73 , 313322 (2018)

To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit

In addition to the development of peptide-based COVID-19 vaccines, industry and academic efforts leverage predicted B- and T-cell epitopes in their subunit vaccines against SARS-CoV-2