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This disruption causes a redox imbalance, leading to membrane lipid peroxidation, reactive oxygen species (ROS) accumulation, and ultimately, iron-dependent oxidative cell death [3]

Use of antioxidants may therefore be a vital factor in sperm survival and function before, during and after cryopreservation

doi: 10.3390/horticulturae8070614 461 MirajSKianiS

The cycle threshold (Ct) values were obtained and 2 (ddCt) was calculated by normalizing with the housekeeping gene B2m (beta-2-microglobulin) for each gene across the PCR arrays to determine fold change

As a result, the gut microbiota can recover to the state that existed before the perturbation, a phenomenon known as resilience (3, 4)