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PBS (177 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 5.5 was used as dilution and running buffer, while PBS (195 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 7.4 was used as regeneration buffer

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After the use of IL-17 inhibitors the immunohistochemical staining also showed moderate intensity staining of inflammatory cells in the lamina propria, but the glandular epithelium of the crypts was widely expressed