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In murine models of breast cancer, ICIE significantly transformed the TME from immunologically cold to hot by diminishing immunosuppressive cells (Tregs, M2-TAMs, MDSCs), augmenting the infiltration of CTLs, and elevating the CD 8+ /Treg ratio

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However, understanding when high B12 levels may become problematic helps ensure safe, evidence-based supplementation under appropriate medical supervision

The samples were centrifuged by 5 min, at 4 C, using a speed 12 000 rpm

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