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To account for potential pleiotropy, in each analysis, we used different analytic methods robust to pleiotropy, including Mendelian randomization pleiotropy residual sum and outlier (MR-PRESSO), a weighted median, and weighted mode

The concentration of the cooling tower water equal to the total mass of contaminates contributed by the water that was evaporated drifted away, and entered the system via blowdown

In both cases, a P value of 0.05 was used to establish statistical significance

Next, the solution was stirred for 10 min under a temperature of 80 C using a magnetic stirrer

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