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Plasmid construction and transfections For over-expression and lentiviral packaging, pCMV-KCNN3-3*Flag, pCMV-Vector, PMD2G, and PSPAX2 were purchased from MiaoLing plasmid platform

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These observations allowed postulating that intracellular redox state of the thiol pool, which strongly depends on GSH level, can drive autophagic response at multiple levels ( Figure 2 )