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Incubate cells at 37, 5% CO 2 , for 15-20 minutes

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In vitro experiments further reveal that Rd rectifies vasopermeability of RVECs in response to high glucose through the reconstruction of adhering, tight and gap junctions

However, in vitro, angiogenic cytokine treatment activates the nuclear translocation of NRF2, which stimulates the tube formation of endothelial cells