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tuberculosis H37Rv strain (ATCC 27294) were cultured in 50 ml of Middlebrook 7H9 (Becton Dickinson, Sparks, MD, USA), supplemented with oleic acid, bovine albumin (Fraction V), dextrose, and catalase (OADC, Becton Dickinson) enrichment and 0.05% Tween 80 (Sigma-Aldrich) contained in a 125-ml Erlenmeyer flask with a plain bottom (Nalgene, 4112-0125, NY, USA)

This is where bioavailability becomes the most important part of the conversation

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[Abstract]2020 Sep 8;11:560170