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Corresponding normal lung or adjacent normal tissues were included in the microarray as negative controls as well

The border of the brain (or veins, heart or pancreas) in the image was marked using ImageJ software and then the surface area of the brain (or veins, heart or pancreas) was measured

Finally, the samples were incubated with primary anti-BrdU (1:100

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FDAs approved indication is that ADCETRIS is indicated for the treatment of adult patients with previously untreated systemic anaplastic large cell lymphoma (sALCL) or other CD30-expressing PTCL, including angioimmunoblastic T-cell lymphoma and PTCL not otherwise specified (NOS), in combination with cyclophosphamide, doxorubicin, and prednisone. The agency took issue with the companys claims that suggest the use of Adcetris for the general treatment of T-cell lymphoma or certain previously untreated CD30-expressing peripheral T-cell, but they do not specify the subtypes in the approved indication