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To this LSB-2, different NaCl concentrations (75, 150 and 300 mM), IGEPAL CA-630 (1%) and increased buffer volume (1250, 1875 and 3750 L, respectively giving rise to ratios of ~ 1.6 10 12 , ~ 1.06 10 12 , ~ 0.53 10 12 cells/ml of buffer) for a 250 ml culture pellet was used along with BugBuster Protein Extraction Reagent and Sonication conditions were tested